Peter Lipke | Biology

Internet Lectures:

Lipke, P. (2015) “Force-induced cell wall amyloid A-D: ale, biofilms, commensalism, and disease" ASM Microbe World. Online at: https://vimeo.com/microbeworld/review/137614592/444908391d

Lipke, P. (2012), "Roles of glycans in yeast cell walls", in Pierce, M. (ed.), Glycobiology: , The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London (online at http://hstalks.com/?t=BL1483198-Lipke)

Klotz, S., and P. Lipke (2010) Medicine Grand Rounds: Candidiasis: Important Clinical and Basic Science Perspectives. https://streaming.biocom.arizona.edu/people/?id=12833

Some Recent Lipke lab Publications:

Some recent papers, with grad student authors in italics and undergrads underlined:

(Click here for PubMed/Entrez listing of old and new papers from our lab )

Medical Consequences of Fungal Surface Amyloids

Candida amyloid adhesins are Romulan cloaking devices:

Behrens, N.E., P.N. Lipke, D. Pilling, R.H. Gomer and S.A. Klotz. Serum Amyloid P Component Binds Fungal Surface Amyloid and Decreases Human Macrophage Phagocytosis and Secretion of Inflammatory Cytokines. 2019. mBio 10:e00218-19. https://doi.org/10.1128/mBio.00218-19. PMC6414697

Klotz, S.A., R. Sobonya, P.N. Lipke, and M.C. Garcia-Sherman. 2016. Serum Amyloid P Component binds to Amyloid in Pathogenic Fungi: Host Protection or a Trojan Horse? Open Forum Infectious Diseases. doi:10.1093/ofid/ofw166 PMC5047411

Garcia-Sherman, M.C., T. Lundberg, R Sobonya, P.N. Lipke, and S.A. Klotz. 2015. A Unique Biofilm in Human Deep Mycoses: Fungal Amyloid is bound by Host Serum Amyloid P Component. NPJ Biofilms and Microbiomes. npj Biofilms and Microbiomes 1, Article number: 15009 (2015) ?doi:10.1038/npjbiofilms.2015.9 [“Featured article”] PMID: 26366292; PMC4563996

Garcia-Sherman, M.C., N. Lysak, A. Filonenko, H. Richards, R.E. Sobonya, S.A. Klotz, and P.N. Lipke. 2014. Peptide detection of fungal functional amyloids in infected tissue. PLoS One http://dx.doi.org/10.1371/journal.pone.0086067. PMC3897640

Candida adhesins and amyloids: a novel type of cell-cell adhesion

Dehullu, J., C. Valotteau, P. Herman-Bausier, M. Garcia-Sherman, M. Mittelviefhaus, J.A. Vorholt, P.N. Lipke*, and Y.F. Dufrêne* (*co-corresponding authors). 2019. Homophilic adhesion by Candida albicans Als proteins is mediated by amyloid-like interactions between cells. Nano Letters 19, 3846-3853 https://doi.org/10.1021/acs.nanolett.9b01010

Dehullu, J., J.A. Vorholt, P.N. Lipke, and Y.F. Dufrêne. 2019. Fluidic force microscopy captures amyloid bonds between microbial cells. Trends Microbiol. 2019 Jul 1. pii: S0966-842X(19)30156-8. doi: 10.1016/j.tim.2019.06.001

Lipke, P.N., S.A. Klotz, Y.F. Dufrene, D.N. Jackson, and M.C. Garcia-Sherman. 2018. Amyloid-like β-Aggregates as Force-sensitive Switches in Fungal Biofilms and Infections. Microbiol. Molec. Biol. Reviews, 82:e00035-17. https://doi.org/10.1128/MMBR.00035-17. [Cover illustration].

Lipke, P.N. 2016. Glycomics for Microbes and Microbiologists (commentary). mBio 7(4): e01224-16 doi:10.1128/mBio.01224-16. PMID: 27507827. PMC4981726.

Rameau, R.D., D.N. Jackson, A. Beaussart, Y.F. Dufrene, and P.N. Lipke. 2016. The human disease-associated Ab amyloid core sequence forms functional amyloids on fungal cells. mBio 7(1):e01815-15. doi:10.1128/mBio.01815-15. PMID: 26758179; PMC4725003

Chan, C.X.J, S. El-Kirat-Chatel, I.G. Joseph, C.B. Ramsook, D.N. Jackson, Y.F. Dufrene, and P.N.Lipke. 2016. Force-Sensitivity in Saccharomyces cerevisiae flocculins. mSphere pii: e00128-16. doi: 10.1128/mSphere.00128-16. PMID: 27547825. PMC4989244

Jackson, D.N., L. Yang, S.B. Wu; E.J. Kennelly, and P.N. Lipke. Garcinia benzophenones promote hyphal apoptosis and potentiate activity of fluconazole in C albicans biofilms. 2015. Antimicrobial Agents Chemother. 59: 6032-6038. doi: 10.1128/AAC.00820-15 PMID: 26195512 PMC4576022

Chan, C. X.-J., I.G. Joseph, A. Huang, D.N. Jackson, and P.N. Lipke. 2015. Quantitative analyses of force-induced amyloid formation in Candida albicans Als5p: activation by standard laboratory procedures. PLoS ONE 10(6): e0129152. doi:10.1371/journal.pone.0129152. PMID: 26047318; PMC4457901

Lipke, P.N., C. Ramsook, M.C. Garcia-Sherman, D.N. Jackson, C.X.J. Chan, M. Bois, and S.A. Klotz. 2014. Between amyloids and aggregation lies a connection with strength and adhesion. New Journal of Science Vol. 2014 Article ID 815102, http://dx.doi.org/10.1155/2014/815102. PMC3981074

Chan, C.X-J. and P.N. Lipke. 2014. Role of force-sensitive amyloid-like interactions in fungal catch-bonding and biofilms. Eukaryot. Cell 13:1136-1142. doi: 10.1128/EC.00068-14. PMC4187618. [cover Illustration]

Bois, M., S. Singh, A. Samlalsingh, P.N. Lipke, and M.C. Garcia. 2013. A role for Candida albicans Als5p amyloid in commensalism in Caenorhabditis elegans? Eukaryot. Cell.12:703-11. doi: 10.1128/EC.00020-13 [cover illustration]

El-Kirat-Chatel, S., A. Beaussart, D. Alsteens, D.N. Jackson, P.N. Lipke and Y.F. Dufrene. 2013. Nanoscale analysis of caspofungin-induced cell surface remodeling in Candida albicans. Nanoscale 5(3):1105-15. doi: 10.1039/c2nr33215a

Alsteens, D., A. Beaussart, S. Derclaye, S. El-Kirat, H.-R. Park, P. Lipke, P., and Y.F. Dufrene. 2013. Single-Cell Force Spectroscopy of Als-Mediated Fungal Adhesion. Analytical Methods 2013 : 3657-3662. DOI: 10.1039/c3ay40473k [cover illustration]

J.J Heinisch, P.N. Lipke, A. Beaussart, S. El Kirat Chatel, V. Dupres, D. Alsteens, and Y.F. Dufrene. 2012. Atomic force microscopy–looking at mechanosensors on the cell surface. J. Cell Sci. In press.

Alsteens, D., C.B. Ramsook, P.N. Lipke, and Y.F. Dufrene. Unzipping of a functional microbial amyloid. ACS Nano in press. DOI:10.1021/nn3025699

Gilchrist KB, Garcia MC, Sobonya R, Lipke PN, Klotz SA. 2012. New Features of Invasive Candidiasis in Humans: Amyloid Formation by Fungi and Deposition of Serum Amyloid P Component by the Host. J Infect Dis. PMID: 22802434 [cover feature and editorial comment]

Lipke PN, Garcia MC, Alsteens D, Ramsook CB, Klotz SA, Dufrêne YF. 2012 Strengthening relationships: amyloids create adhesion nanodomains in yeasts. Trends Microbiol. 2012 Feb;20(2):59-65. PMID: 22099004 [cover feature]

Garcia, M.C., J.T. Lee, C.B Ramsook, D. Alsteens, and Y.F Dufrene. 2011. A role for amyloid in cell aggregation and biofilm formation. PLoS One 6: e17632. doi:10.1371/journal.pone.0017632

Alsteens, D., M.C. Garcia, P.N. Lipke*, and Y. Dufrene* (*co-corresponding authors). 2010. Force-induced formation and propagation of adhesion nanodomains in living fungal cells. Proc. Natl. Acad Sci., USA 107:20744-9. [featured “In This Issue”]

Frank, A.T., C. Ramsook, H.N. Otoo, C. Tan, G. Soybelman, J.M. Rauceo, N.K. Gaur, Stephen A. Klotz, and P.N. Lipke. 2010. Structure and Function of Glycosylated Tandem Repeats from Candida albicans ALS Adhesins. Eukaryot. Cell 9: 405-414. [Cover illustration]

Ramsook, C., C. Tan, M.C. Garcia, R.Fung, G. Soybelman, R. Henry, A. Litewka, S. O’Meally, H.N. Otoo, R.A. Khalaf, A.M. Dranginis, N.K. Gaur, S.A. Klotz, J.M. Rauceo, C.K. Jue, and P.N. Lipke. 2010. Yeast Cell Adhesion Molecules Have Functional Amyloid-Forming Sequences. Eukaryot. Cell 9: 393-404. [“Spotlighted“ Article; ASM Microbe “Highlight”]

Alsteens, D., V. Dupres, S. A. Klotz, N.K. Gaur, P.N. Lipke, and Y.F. Dufrêne. 2009. Unfolding Individual Als5p Adhesion Proteins on Live Cells. ACS Nano 3:1677-1682.

Klotz, S.A., N.K. Gaur, R. De Armond, D. Sheppard, N. Khardori, J.E. Edwards, Jr , P.N. Lipke, and M. El-Azizi. 2007. Candida albicans Als proteins Mediate Aggregation with Bacteria and Yeasts. Medical Mycology 45: 363-370.

Otoo, H.N. K.G. Lee, and P.N. Lipke. 2008. Candida albicans Als adhesins have conserved amyloid-forming sequences. Euk. Cell 7: 776-782 [Cover Illustration for Eukaryotic Cell and Highlighted in ASM Microbe]

Dranginis, A., J. Rauceo, J.E. Coronado, and P.N. Lipke. 2007. A biochemical guide to yeast adhesins: glycoproteins for social and antisocial occasions. Microbiol Mol Biol Rev. 71:282-294.

Candidiasis

Thorn, J. L., K.B. Gilchrist, R.E. Sobonya, N.K. Gaur, P.N. Lipke, and S.A. Klotz. 2010. Postmortem Candidemia: Contamination or Evidence of Disseminated Disease? J. Clin. Pathol. 63:337-340

Klotz, S.A., B. Chasin, B.Powell, N.K. Gaur, and P.N. Lipke. 2007. Polymicrobial Bloodstream Infections Involving Candida species: Analysis of Patients and Review of the Literature. Diag. Microbiol. Infect. Dis. 59: 401-406.

Spellberg, B. A.S. Ibrahim, L. Lin, V. Avanesian, Y. Fu, P.N. Lipke, H.N. Otoo, T. Ho, and J.E. Edwards Jr. 2008. An antibody titer threshold predicts anti-candidal vaccine efficacy even though the mechanism of protection is induction of cell-mediated immunity. J. Infect. Dis. 197:967-971. Featured article

Spellberg, B., AS Ibrahim, MR Yeaman, L. Lin, Y Fu, V Avanesian, AS Bayer, SG Filler, P.N. Lipke, H.N. Otoo, and J.E. Edwards Jr.

2008. The anti-fungal rAls3p-N vaccine protects mice against the bacterium Staphylococcus aureus. Infect Immun. 76: 4574-4580.

Evolution

Xie, X., W. Qiu, and P.N. Lipke. 2011. Accelerated and adaptive evolution of yeast sexual adhesins. Mol. Biol. Evolution. 11:3127-37

Xie, X., and P.N. Lipke. On the Evolution of Yeast and Fungal Cell Walls. 2010. Yeast 27:479-488.

Coronado, J.E., O. Attie, S.L.Epstein, W.-G Qiu, and P.N.Lipke. 2007. Discovery of Recurrent Sequence Motifs in Saccharomyces cerevisiae Cell Wall Proteins. MATCH Commun. Math. Comput. Chem. 58:281-299.

Coronado, J.E., S. Mniemneh, S.L. Epstein, W. Qiu, and P.N. Lipke. 2007. Conserved Processes and Lineage-Specific Proteins in Fungal Cell Wall Evolution. Eukaryotic Cell. 6: 2269-2277.

Cell walls

Gonzalez, M., N. Goddard, C. Hicks, R. Ovalle, C.K. Jue, and P.N. Lipke. 2010. A Screen for Deficiencies in GPI-Anchorage of Wall Glycoproteins in Yeast. Yeast 27: 583-596.

Gonzalez, M., P.N. Lipke, and R. Ovalle. 2009. GPI Proteins in Biogenesis and structure of yeast cell walls. In The Enzymes vol 26, Glycosylphophatidylinositol (GPI) anchoring of proteins, eds. A.K. Menon, T. Kinoshita, P. Orlean and F. Tamanoi, Academic Press/Elsevier pp. 321-356.

Gonzalez, M., P.W.J. de Groot, F.M. Klis, and P.N. Lipke. 2008. Glycoconjugate Structure and Function in Fungal Cell Walls. In A. oran, P. Brennan, O. Holst and F. von Itzstein (eds.) Microbial Glycobiology: Structures, Relevance, and Applications. Elsevier.

Patents:

Jackson, D.N., Kennelly, E., and P.N. Lipke. Antibiofilm composition comprising Garcinia extracts. U.S. Patent #9.9648,876 B2 issued 5/16/17

Lipke, P. and U. Edupaganthi. Process for increased yeast biomass. Patent Application 15/856,667

Click here for PubMed/Entrez listing of old and new papers from our lab